Under a microscope, if the gram stain is purple, the actinium is gram positive, if the stain is red, it is gram negative. The next test was the fermentation tests for glucose, sucrose and lactose. If an organism is able to ferment the carbohydrate, there will be acid produced, which will turn the broth yellow. Homogeneities shows that only one product was produced- acid. Other organisms will carry out heterogeneity’s, the presence of acid and gas. If the organism is unable to ferment the sugar in the tube, there will be no change in the orange/red color of the broth and will be considered alkaline.
The following tests that I performed were tests for methyl red and vogues prosperous in SIMI media. A positive methyl red test indicates a mixed acid fermentation and would turn the organism’s broth red, if no color presents, it is negative. A positive vogues prosperous test indicates fermentation to neutral products and will turn the broth red, if no color presents, it is also negative. Stab inoculating the SIMI media for these tests will also show if the organism produces sulfur or indolent and if it is motile.
If sulfur is present, then the medium will be lack, if it produces indolent, the medium will be red, and if it is motile, there will be growth throughout the tube. Also grew the organism on McCracken agar. After inoculation, visible colonies may or may not be present. Objective: The objective is to identify the unknown organism. Hypothesis: If I perform several different tests, then I will identify the unknown bacterium. Materials/Procedure: The following tests according to the lab manual were performed: gram stain, fermentation tubes, methyl red, vogues prosperous, sulfur, indolent, motility and rowing it up on McCracken agar.
The gram stain was performed incorrectly the first time. This is because the decolonize was not on the bacterium slide for long enough, giving a false outcome. Results: I TESTS I Gram Stain I Acid/Gas I Grew Discussion: I RESULTS Ciscoes Fermentation I I Lactose Fermentation I I Sucrose Fermentation I I Methyl Red I Vogues Prosperous Sulfur lend I Motility McCracken Agar The hypothesis was supported. For the gram stain, my organism turned red, meaning that my bacterium was a gram negative and it was also rod shaped.
After this test, the possible organisms were: Entertainer arrogates, Escherichia coli, Seriate marches, Cacciatore friendlier, Protest miracles, Pseudopodia organisms, Pseudopodia fluorescent, Salmonella or Shillelagh. The fermentation tubes were all heterogeneity; glucose, sucrose and lactose all turned out to be acid/gas. This narrowed the list down to these possible organisms: Entertainer arrogates and Cacciatore friendlier. To determine between the two, I did the methyl red test next. The methyl red test was negative, meaning the pH was above 6 and leaving only one organism: Entertainer arrogates.
To confirm this, the Vogues Prosperous test was performed. The Vogues Prosperous test detects action by oxidation with oxygen in the presence of KOCH. These results were positive. The sulfur, indolent and motility tests were also confirmations. The bacterium contained no sulfur, did not produce indolent and was motile. It also grew on McCracken. My bacterium is definitely Entertainer arrogates. Real World Importance: Entertainer arrogates is responsible for many monoclonal, opportunistic pathogens causing infections. It can also cause different types of community acquired infections.
Most people who acquire an infection from this bacterium already have an existing condition, meaning they are miscomprehended- making them more susceptible to these infections. These include patients who are staying in the hospital, especially in the intensive care unit for long periods of time. Some strains of E. Arrogates are resistant to treatments, which is why they can colonize in hospital settings. But the majority of its strains are sensitive to most antibiotics. Some of the infections caused by this organism are from Avis, theaters, surgeries and specific antibiotic treatments.
This species is found in the human intestinal tract as normal microbial but if it is transmitted to other parts, it can cause skepticism and urinary tract infections. E. Arrogates is not always found in the intestine, but is also found in water and sewage. It does not usually cause any disease unless, as previous stated, the patients have an underlying condition. The mortality rates of E. Arrogates are not high, but it depends on the severity of the underlying conditions. The infections are most moon in babies and in elders.