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Some of the various methods introduced and practiced in class were applied in identifying the microbe. Procedures were followed as stated in the index of the Virtual Unknown Software. First the shape and color of the microbe was noted. This is done to know the morphology of the microbe. After concluding that the microbe was Gram negative rods, it was inoculated into a previously prepared test tube. Then various tests were performed to isolate and identify the unknown microbe. Table 1 shows the tests, purpose, reagents and results of the various tests performed.

Each test was performed according to the index of the Virtual Unknown software and the following tests were performed: 1) Donation Fermentation 2) Cellulose Fermentation 3) Maltose Fermentation 4) Lactose Fermentation 5) Refines Fermentation 6) Servitor Fermentation 7) Sucrose Fermentation 8) Melodies Fermentation 9) Indolent Production Results Table 1: Biochemical Tests Results Test I Purpose I Reagents I Observations I Results I Donation Fermentation I To determine if the microbe can ferment the carbohydrate (sugar) donation as a carbon source. I None I Turned pink I

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Negative donation test I Cellulose Fermentation I To determine if the microbe can ferment the carbohydrate (sugar) cellulose as a carbon source. I None Turned Yellow I Positive cellulose fermented I Maltose Fermentation I To determine fifth microbe can ferment the carbohydrate (sugar) maltose as a carbon source. I None I Turned Yellow I Positive maltose fermented Lactose Fermentation I To determine if the microbe can ferment the carbohydrate (sugar) lactose as a carbon source. I None I Turned Pink I Negative lactose fermented I Refines Fermentation To determine whether the microbe can SE sugar refines for carbon and energy.

I None I Turned pink Negative refines fermented I Servitor Fermentation I To determine if the microbe can ferment the carbohydrate servitor as a carbon source. I None I Turned yellow I Positive servitor fermentation I Sucrose Fermentation To determine if the microbe can ferment the carbohydrate sucrose as a carbon source I None I Turned Pink I Negative sucrose fermented I Melodies Fermentation To determine if the microbe can ferment the carbohydrate (sugar) melodies as a carbon. I None I Turned Pink I Negative melodies fermented I Indolent

Production I To determine whether the microbe can produce indolent from the amino acid thyrotrophic. I Add five to ten drops of Kava’s reagent to the test tube. The reagent does not mix with water and forms a thin layer above the broth. The reagent reacts with idle to produce a cherry red ring. I Red ring at the top of broth I Positive indolent test I Flowchart Unknown#l Gram Stain Gram Negative Rod Donation Test (-) Positive Negative Kielbasa ornithological Cacciatore kosher Kielbasa exotica Interchangeableness Escherichia Ferguson Kielbasa pneumonia SSP Kielbasa ornithological

Kielbasa pneumonia pneumonia SSP cozenage Kielbasa detergent Kielbasa pneumonia SSP Lacier declarations aromatherapies Kielbasa detergent Lacier declaratory Molecular wooziness’s Providence allegiances Providence retire Seriate functional Serial reburied Cellulose fermentation (+) Butterball aggregates Interacted cloacae Interacted intermediates Interacted sukiyaki Cleaver ascorbic Renal aquatints Angelina Americana Moreland inorganic SSP marginal Edwardians hosanna Edwardians catalytic Edwardians tardy Edwardians tardy vigorous 1

Escherichia palatable Escherichia coli Moreland inorganic SSP subsonic Protest memorabilia Protest penned Providence rusticating Salmonella Bangor cholera’s Salmonella paratyphoid A Seriate marches proclamations Shiningly dysentery’s Shiningly sonnet Yearnings pests Maltose Fermentation (+) Protest monomaniacs Protest vulgarism Providence Stuart Salmonella SSP Arizona Salmonella typhoid Seriate Shiningly proclamations Shiningly flexible Oatmeal potatoes Yearnings pseudoscience’s None Lactose Fermentation (-) Positive Negative Butterball aggregates Cacciatore implications vigorous 1

Interacted cloacae Interacted amusingness vigorous 1 Interacted vultures Interacted sukiyaki Renewal aquatically Seriate adenosine vigorous 1 Seriate polyatomic Refines Fermentation (-) Citrate implications vigorous 1 Interacted amusingness vigorous 1 Cadence advise Cadence leafage Interacted groovier Interacted cancerous Escherichia vultures Escherichia henchman Seriate odoriferous vigorous 1 Hafting alive Yearnings criterion Servitor fermentation (+) Cadence netter Cadence advise Seriate official Yearnings intervocalic Yearnings Frederickson Hafiz alive Yearnings Christiansen

Cedar leafage Interacted cancerous Escherichia Hermann Sucrose fermentation (-) Ceased netter Interacted amusingness vigorous 2 Seriate official Salmonella choristers SSP hotplate Yearnings intercalation Melodies Fermentation (-) Interacted amusingness vigorous 2 Cacciatore Freddie Salmonella cholera’s SSP hootenanny Cacciatore implications Indolent production (+) Cacciatore implications Discussion/Conclusion Cacciatore friend After conducting a variety of various tests, it was concluded that Unknown #1 was Cacciatore implications.

Once the morphology of the microbe was identified, it was isolated and inoculated into a test tube. The nine tests performed on the microbe were done according to the index of the virtual unknown software and the results of each test correlated to the results anticipated. As a result, it was concluded that the unknown #1 microbe was Cacciatore implications. Cacciatore implications has a gram negative rod-shape. They can be found in the digestive system and are linked with digestive disorders such as diarrhea. They can also be present in the urinary tract and most commonly causes Urinary Tract Infections.

Cacciatore implications is also known as an opportunist pathogen. Opportunist pathogens are pathogens that do not cause much damage to healthy individuals; however, it can cause very severe damage to those with a weakened immune system. Besides being found in the intestines of humans, Cacciatore implications is usually found in the ground (soil), in the air and in the intestines of animals such as bats. (1) Cacciatore implications also has some positive effects. It has the ability to recycle hydrogen. In addition it is capable of breaking down nitrates into nitrites in the nitrogen cycle.

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