Identifying C. Friendlier holds importance in order to determine infection in gastrointestinal and urinary tracts and in the brain. These infections will often be noticed through pain in these areas. The main purpose of this laboratory experiment was to utilize microscopic and macroscopic observation, biochemical tests, and antibiotic and disinfectant sensitivity tests to identify an unknown bacterium. Materials and Methods Microscopic and Macroscopic Observations All samples and observations of the unknown bacterium were taken after 48 hours of incubation at 350 Celsius.
Microscopic observations were performed on four different microscope slides. Three of these observations were gram stains and the last observation was of an endoscope staining. The unknown bacteria samples for the endoscope stain were taken off of a nutrient agar plate and stained using the Bartholomew and Emitter’s method. Gram stain samples were taken off of tropic soy agar and stained using the gram stain procedure that is listed in the Willing and Bleakly manual (2). Physiological Tests All biochemical tests are shown in table 4.
Each of these tests required an incubation time of 48 hours at an incubation temperature of 350 Celsius with an exception to the gelatin test that required a 30 minute refrigeration period before observing results. All tests were performed according to the procedures listed in the Willing and Bleakly lab manual (2). Antibiotic and Disinfectant Tests The sensitivity or irresistibility of C. Friendlier to antibiotics and disinfectants was tested using the steps outlined in the Willing and Bleakly lab manual (2). The four tested antibiotics include: penicillin G, 10 U; chlorination, 30 pm; tetracycline, 30 pm; and inclining 10 pm.
The four disinfectants that were tested on the unknown bacteria include: Lasso, Basic G, Colors, and Assist CT. Susceptibility of irresistibility of the unknown bacteria to these antibiotics and disinfectants were tested and measured using the chart on page 1 25 and the Kirby-Bauer test procedures in the Willing and Bleakly lab manual as a reference (2). These results are shown in table 3. Data The following results show that C. Friendlier had the following colonial morphology when observed in an isolated pure culture (Table 1). Observation C. Friendlier Colonial Morphology Form Circular
Elevation Raised Margin Undulate Size Medium Color Beige Texture Mucous Picturesque The following results indicate that C. Friendlier is a facultative anaerobe and a gram negative rod shaped bacterium that is endoscope negative and motile (Table 2). Observations Stain, Oxygen, and Motility Test Results Gram Stain Pink and rod shaped Gram negative rods Endoscope Stain No green spots Endoscope negative Technologically Test Growth in both areas Facultative anaerobe Motility Fuzzy, red growth Motile bacterium The next results indicate the susceptibility or irresistibility of C.
Friendlier to antibiotics and disinfectants (Table 3). Antibiotic/Disenfranchisement Inhibition Diameter POI norm R DEBT Mom R CO mm S AMINO mm S Lasso Mom R Basic G mm R Colors mm R Assist Stem R (POI=Penicillin G, CO= Chlorination, TOTE=Tetracycline, AMINO=Inclining, S=susceptible, R=Resistant) Table four can be found on the next page and it shows the biochemical test results for C. Friendlier. All results are consistent with the documented results found in the Willing and Bleakly lab manual (2). Test Observation Result Glucose Yellow/ bubble Positive Sucrose Red Negative Lactose Red Negative
Imitation Yellow w/ bubble Positive Indolent Yellow ring Negative Methyl Red Red Positive UP Brown Negative Citrate Hydrolysis Blue Positive Areas Yellow Negative Gelatin Solid Negative Catalane Bubbles on slant Positive (UP=Vogues-Prosperous) Results The unknown bacteria formed colonies that appeared circular, undulate, medium in size, raised in elevations, opaque, mucous in texture, and beige in color as shown in table 1. Table 3 shows Gram stain results that indicated C. Friendlier as a gram negative bacterium in rod shapes scattered in singles and some in pairs.
Each gram stain produced the same results. The Bartholomew and Emitter method of endoscope staining indicated that C. Friendlier tested negative for endoscope formation. Table 4 shows the biochemical test results of the unknown and the official test results for comparison. The Kirby-Bauer antibiotic tests indicated that the unknown is susceptible to chlorination and inclining and resistant to penicillin G and tetracycline. The disinfectant sensitivity tests indicated that C. Friendlier is resistant to all four of the disinfectants.
Discussion The biochemical tests, the Gram stain, the Bartholomew and Emitter’s stain, the gyroscopic and macroscopic observations, and chemical sensitivity tests helped to identify the unknown bacteria as Cacciatore Friendlier. Each biochemical test result maintained consistency with the official recorded results in the Willing and Bleakly lab manual (2). Chemical tests showed which antibiotics would be most effective in treating a C. Friendlier infection. Penicillin and Tetracycline would be poor choices for treating C. Friendlier infection due to the fact that C. Friendlier is resistant to these antibiotics. However, C.
Friendlier is incredibly susceptible to Chlorination and also significantly susceptible to Inclining. These antibiotics would therefore be effective in treating a C. Friendlier infection in a patient. Disinfection of C. Friendlier should not be performed using any of the disinfectants that were tested. This is due to the fact that C. Friendlier is resistant to Lasso, Basic G, Colors, and Assist CT since none of these disinfectants created a large growth inhibition zone. If one of these four disinfectants had to be chosen, Colors would be the most effective as it produced the largest growth inhibition zone, but C.