This is because silica gel is very polar and methyl chloride is slightly polar but it is mostly non-polar which allow the compound to move up the silica gel. Methanol did not work well with separation cause methanol is polar and polar likes polar which cause no separation. Hexane worked fairly well with two compound separation dues to hexane being very non-polar which allow the compound to separate twice instead of three times. Hence Methyl chloride is the best solvent for separation in the next part of this experiment.Procedure Used three eluting chambers with moll and mm’ beakers and used filter paper to line the beakers then added enough hexane to the beaker to cover the bottom. In each beaker the same procedure was performed. Covered the beakers with foil (to avoid evaporation).
Gathered three small TTL plates (making sure to only touch the edges of the TTL plates to avoid contamination). Placed the plates under a iv lamp to check for contaminates that would affect the experiment.Used a pencil to draw a light line on all three TTL plates about mm up from the bottom and drew a little ax’s in the middle on the line on each of the TTL plates. Then used capillary tubes to dot a compound on each of the TTL plates (on the x). Let the compounds evaporate then used the IV lamp to make sure there was enough solvent on the plate (the dot turned pink). Then placed the TTL plates n there respective chambers (covered beakers) and recovered the beakers. Then waited for the solvent to travel approximately 3/4 up the TTL plate or until the solvent stops moving.When the solvent reached % of the way up the TTL plate the plates were taken out of the chamber and a line was drawn when to mark how far the solvent traveled.
Let the solvent dry and used a IV lamp to see the circles. Circled the spots with a pencil when there are three circles on the TTL plate it was considered a good eluting solvent for was used in the further experiment. With the correct solvent used a large TTL plate and a line in pencil mm up from the bottom and drew four ax’s on the line (above the ax’s towards the top of the TTL plate labeled the compounds respectively).Used the IV light to check for impurities and placed a spot of compound on three of the ax’s then placed a spot of unknown B compound on the last x (with capillary tubes).
Made sure there was enough solvent on the TTL plate by using the IV lamp (dots turned pink). Eluted the TTL plate in a 400 beaker with the methyl chloride (enough to line the bottom) and waited for the solvent to reached 3/4 up the TTL plate. Then removed the plate and marked how far the solvent traveled and aided for the solvent to dry.
Then used the IV lamp to circle the separation points.The identity of the compound of the compound could be determined based on the separation circles. Discussion: A short discussion of the results and outcome of the experiment, including TTL analysis.
In this section, you should explain what was accomplished during the experiment, how it was accomplished and how well it was accomplished. A discussion of the theory of chromatography and TTL as it applies to this experiment should be given. Any major sources of error should be discussed. Any other pertinent information should be given in this section.