The measurements were taken by observing where the agar cleared around the solutions, as the agar was cloudy where bacteria was present. I hypothesized that saliva would have the greatest effect, as the mouth is full of bacteria. Thought the stock solution would be the second most effective, as it is a simple solution of lissome. I predicted that Mucus would be third, as the nose has a similar environment to the mouth, and tears would be less effective, as I saw them as more of a lubricant than a losing solution. I thought that distilled water would observe no effect as it has no lissome.
Methods The class, which was previously separated into groups of around four people each, was distributed pitter dishes containing agar with freeze-dried Microcosmic Lutes cells for testing. Each group was also given a microcomputer, distilled water, paper discs for marking purposes (all courtesy of the Milliamp College Botany Lab) and a stock solution (containing 1 MGM of lissome per ml, provided by Carolina Biological in Burlington, NC). The individual groups had to produce the other three solutions, mucus, saliva, and tears, to be tested in the agar.
Each f the five solutions (distilled water, stock solution, saliva, mucus, and tears) were measured to 20 micrometers and placed on the paper discs which were color coded in order to keep notes of the varying solutions. The Paper discs were spaced out along the pitter dish containing the agar, and the dishes were set aside for further testing. Measurements of the distance of clarity observed per each solution were taken 2 hours later, then 24 and 48 hours later. Each group had to observe the effects of the solutions on the pitter dishes for every dish that was prepared.
The distance of clearing was measured in millimeters to test the strength of the lissome in each solution. Results Out of the five pitter dishes that were prepared, only two provided usable data; the three dishes that were unreadable had misreported agar, in which the bacteria had previously formed clumps and did not distribute sufficiently in the agar. The remaining three dishes for groups one, two, and five provided varying data: Group One’s solutions observed effects for just the stock solution and the tears. After two hours, the stock solution observed no losing, while the tears already had five mm of clearance.
After 24 hours, the stock solution showed 5 mm of clearing while the tears showed 14 mm. After 48 hours, the stock solution lased to a distance of 12. 5 mm away from the disc while tears measured 18 mm. For Group Two, it seemed that all of the solutions were correctly prepared. After two hours, however, no solutions observed any losing. After 24 hours, saliva had cleared 7. 5 mm, the stock solution had cleared 10 mm, cuscus 7. 5 mm, sears 15 mm, and stilled water 0 mm. After 48 hours, saliva cleared 10 mm away from the disc, the stock solution 12. Mm, mucus 10 mm, tears 17. Mm, and distilled water still O mm. The averages between the two successful pitter dishes were also observed. After two hours, the only solution that observed any losing was tears, at an average of 2. 5 mm. After 24 hours saliva averaged 3. 75 mm, the stock solution 7. 5 mm, mucus 3. 75 mm, tears 14. 5 mm, and distilled water O mm. The averages at 48 hours between the two groups observed a clearance of 5 mm for saliva, 12. 5 mm for the stock solution, 5. 25 mm for mucus, 17. 75 mm for tears, and still O mm for distilled water (observe Figure 1 for all measurements).