Because of the presence of perspicuous flagella, species included this genus are motile and most strains swarm in periodic cycles, producing a characteristic concentric swarming pattern, or parade in a uniform film over nutrient media solidified with agar or gelatin, which is evident in the streak plates done over the course of a few weeks, the streak plates showing the bacteria spreading throughout the entire agar plate with a hazy, cloudy appearance. Motility of the unknown was also proven through the indirect method of inoculating Motility S medium.
Like other members of the family Intercontinental, it is also facultative anaerobic, and the unknown culture was proven to be as such through the use of Fluid Technologically Broth, or FAT medium. The genus Protests is known to catabolic glucose and a few there carbohydrates, with the production of acid and usually gas. As for the unknown, it was able to ferment glucose (without the production of gas, as the Durham tube placed inside the PR Glucose was not displaced) and sucrose, but not lactose.
The abovementioned genus is also indicated to produce a negative result in oxides test and a positive result in catalane test, and the unknown produced negative and positive results in those tests, respectively. The genus is also indicated in the Vergers manual as having a positive result in the methyl red (MR..) test; however, the unknown initially produced a negative result (after adding 8-10 drops of the MR.. Indicator, the unknowns color changed to a yellow-orange result) which is the sole discrepancy in this report.
However, it is possibly important to note that after a short while, the contents of the tube containing the MR.. Test changed back to a shade of red, possibly indicating that the culture is indeed methyl red positive. Other notable characteristics of this genus are the following: species vary results in indolent, Vogues-Prorogues, and Simmons citrate test; Protests produces negative result in lysine destroyable and a positive result in both phenylalanine dominate and areas test; HAS s usually produced; and gelatins production is usually positive.
A series of tests must be conducted to further eliminate other candidates for the identity of the unknown organism, this time to identify the unknowns species name. With the use of Vergers Manual of Determinative Bacteriology, such tests are indicated as follows: indolent test, Vogues-Prorogues test, cytosine displeasure test (HAS production), test for Ernestine destroyable, acid production in maltose and D-Otiose. According to Burgers Manual, out of the four species in the genus Protests, only Protests vulgarism indicates a positive result for the indolent test.
The unknown culture had a negative result for the indolent test, thus eliminating the possibility of Protests vulgarism as the possible identity of the unknown culture. The Vogues-Prorogues (UP) test results for species in the Protests genus also differ, and this would possibly eliminate one or two species. Because the UP test result of the unknown is positive, it eliminates Protests vulgarism once again, as well as Protests pioneer, which both have negative results in the UP test. To further eliminate other species, the hydrogen sulfide (HAS) production test using Selfless Iron Agar (AKA) slant may be used.
The unknown had a positive exult for HAS production, and as such, eliminated Protests monomaniacs from the possible identities of the unknown, thus bringing down the possible identity of the unknown organism into one: Protests memorabilia. According to Burgers manual, only Protests memorabilia discombobulates Ernestine, resulting in a positive Ernestine result. Ernestine is a part of the various tests contained in the Underwrote II test, which was done last in the identification of the unknown organism in the laboratory work.
The Underwrote II system inoculated with the unknown organism showed a positive result for the Ernestine test, which rather establishes the unknown organism’s identity as Protests memorabilia. Furthermore, the code that resulted from the system was either 23007 or 22007, due to a discrepancy with the HAS test in the Underwrote II tube (there was black precipitate present in the tube; however, the coloration in the medium after inoculation and incubation was brown, which the laboratory manual, entitled Bonbon’s Microbiological Applications, indicated should not be confused with true HAS production).
Given this dilemma, it was decided by this student to look through both organisms labeled under 23007 and 22007. These two odes only named one sole organism, which was, again, Protests memorabilia. The abovementioned results taken led this student to believe that the identity of unknown number 4 is Protests memorabilia. Other tests that may be used are the following: acid production in maltose is present (positive) for all species of Protests except for Protests memorabilia, which has a negative result for the said test.
The D-Close acid production test also results in a positive test for all Protests species except for Protests monomaniacs, and thus can be used to eliminate the said test. The Protests genus may be found in intestines, colon and perineum f humans and also a wide variety of animals, and may also occur in manure, soil, sewage, as well as polluted waters. The genus is also becoming increasingly associated with pediatric illness, including neonatal meningitis and pediatric urinary tract infections, but infections of other organ systems have been described.
Most cases of central nervous system infection caused by Protests occur in neonates, and contiguous spread to the brain from localized infections is reported occasionally. According to a journal article published in the Journal of Medical Microbiology, Protests memorabilia is an important pathogen of the urinary react, and is the primary infectious agent in patients with indwelling urinary catheters. Furthermore, individuals suffering from urinary tract infections caused by P. Rabbis often develop bacterium, cystitis, kidney and bladder stones, catheter obstruction due to stone encrustation, acute polytheists, and fever. Also, Protests memorabilia features more prominently as a urinary pathogen for boys than girls, most especially uncircumcised boys. Aside from boys, Protests memorabilia targets young females infrequently, individuals with structural abnormalities of he urinary tract, and the elderly.