Test were performed to determine which Staphylococci was present and the results of the test indicated that Staphylococcus epidermises was present. Introduction: The genus Staphylococcus includes more than 20 species as described in Burger’s Manual (2001). Only two are significant to humans, Staphylococcus erasures and Staphylococcus epidermis. S. Erasures colonizes mainly the nasal passages, but it may be found regularly in the oral cavity, gastrointestinal tract and the skin. Staphylococcus epidermis is an inhabitant of the skin. Of these two Staphylococcus erasures is serious pathogen.
Staphylococcus epidermis is of the normal flora and is not considered to be a serious pathogen (textbookofbacteriology. Net). Staphylococcus epidemics becomes pathogenic hen the skin is broken or through contamination from medical procedure. Staphylococcus erasures are only able to invade via broken skin or mucous membranes. Staphylococcus erasures is very heat resistant and reheating foods will not destroy it, although the bacteria may be destroyed (www. Gunnysack. Due). About 30 per cent of healthy humans are carriers of S. Erasures, they have the bacteria on their skin without any active infection or disease.
Staphylococci hide their antigens to avoid an immune response many different ways to include killing infection-fighting cells (phagocytes), surviving within host infection-fighting ells, and developing a resistance to antibiotics determent. Org) Purpose & Results: Genus Tests l. Gram Stain: a. The purpose of the Gram stain is to confirm that the isolate(s) have the physical characteristics of Staphylococci. B. If the isolate is a Staphylococci it will be a Gram positive Cisco, which will be purple in color and rod shaped, arranged in clusters. C.
After performing the Gram stain on the isolates from the quadrant streak, the isolate were found to be Gram positive, which is positive for Staphylococci. Cluster duplicitous cuscus d. A smear was created from a pure culture of Staphylococcus. It was hoses from the throat one DNA agar. The isolate was gram stained and viewed under oil immersion. The Cisco were purple, which indicates a gram positive. Staphylococci divide in various directions forming grape-like clusters, single Cisco, or just a few together. II. Catalane-explain why it bubbles e. The purpose is to confirm weather strep or stash f.
After putting a drop of peroxide onto isolate it is expected to bubble. G. After applying the peroxide to isolate it did bubble up to indicate Staphylococci. Species Tests Ill. Imitation Fermentation: h. The purpose-To grow and isolate colonies from nose and throat and preferential between the two specie. This is done by performing a quadrant streak on a inanimate salt augur medium to isolate bacteria from the nose and throat and identify as Staphylococcus erasures or Staphylococcus epidermis Expected results-To have isolated colonies surrounded by yellow which is indicative of imitation fermentation.
Staphylococcus erasures imitation fermentation produce acid results in the media tuning it yellow. There is no fermentation in Staphylococcus epidermis, so media does not change, the medium remains red. J. Your results-No imitation positive colonies were resent, therefore no imitation fermentation developed. If positive colonies were present there would have been a change in the color of the colonies to yellow or red/pink. K. Throat and nose quadrant streak Nose quadrant streak on Imitation Salt Agar Negative SE No color change Throat quadrant streak on Imitation Salt Agar Throat quadrant streak Negative SE.
No color change IV. Beta Hemolytic: l. The purpose is to determine if bacteria produce red blood cell-losing extensions or hem-lysine, Beta hemolytic. Beta hemolytic occurs when the bacterial toxins completely else the red blood cells in the medium. Confirm genus of isolate s Staphylococcus and identify species of isolate as Staphylococcus erasures or Staphylococcus epidermis. M. It is expected for beta hemolytic to be present which presents a yellow halo around bacterial growth. Staphylococcus erasures will display this yellow halo effect. N.
My results showed no halo or golden ring around any bacteria on blood agar plate. The result is negative for Staphylococcus erasures. Blood agar Nose isolate (IN from MASS Throat isolates (TTL-TO) from MASS Staphylococcus epidermis Grayish colonies Staphylococcus erasures Golden pigmentation o. One isolated colony was picked from imitation sat’ augur quadrant treats and transferred to blood agar. The isolates were tested for their ability to produce red blood cell losing extensions or hem lysine. The results showed no halo or golden ring around isolates. The isolates show a positive result for Staphylococcus epidermis.
V. Dense: p. The purpose is to evaluate the results of the imitation fermentation form the quadrant streaks to identify potential staphylococci erasures. Q. Expected results-Dense negative destroys DNA. Staphylococci erasures is Dense positive and Staphylococci epidermis is Dense negative. Need more info. R. Your results-white/gray colonies, resulting in Staphylococcus epidermis. Dense agar Nose isolate (IN -NO) from MASS Golden in color with halo Grayish color s. Six isolated colonies were picked from MASS quadrant streaks and transferred to the DNA agar.
The isolates were tested for their ability to produce the economy deoxyribonucleic (Dense). The results showed no halo or golden ring around isolates. The isolates showed positive results for VI. Gold Color: t. The purpose is to indicate whether Staphylococcus erasures or Staphylococcus epidermis is present. U. The results are expected to have a golden color around the Staphylococcus erasures. V. The results were negative or Staphylococcus erasures, therefore positive for Staphylococcus epidemics. The isolates were grayish in color. VI’. Coagulate: w. The purpose is to evaluate results of coagulate test.
Perform antimicrobial susceptibility test on Staphylococcus isolate. Coagulate test is positive for Staphylococcus erasures and negative for all other Staphylococci. X. The expected results are a coagulate-negative Staphylococci (CoNS). Y. The actual results were that the tube of rabbit plasma stayed as a liquid, there was no coagulation of the plasma. VIII. Antimicrobial Susceptibility: z. The purpose is to evaluate results of antimicrobial susceptibility test The results were mostly sensitive, therefore the bacteria was sensitive to these antibiotics. The zones were rather a large area. L. Data Table Antibiotic Disk I Barber.