Before 1673, microbes were unknown ND people seemed to be dying from unknown causes. It was in this year that Anton van Leeuwenhoek was the first person to see and identify living bacteria (Tractor, 2010). Since this amazing discovery, very extensive research has been done on these tiny organisms and currently a great deal of information is known about them. The lab I am reporting on examines different sources of bacteria. How can these bacteria get on the human body and be problematic?
Methods and Materials The design of this lab experiment is to isolate bacteria from any location or item thou getting bacterial contamination from unwanted places. Microorganisms are found in dust particles suspended in the air or and on nasal secretions that are a result of sneezing (Department, 2009). Materials needed are: 100 ml of melted nutrient agar, five sterile Petri plates, and sterile cotton swabs. The first step is to pour nutrient agar into each of the five plates making sure to use aseptic techniques. Allow this to solidify.
While waiting, give each plate one of the following labels: sterile, 1, 2, 3, 4. The sterile plate will be the control. Nothing will be done with this plate to make sure that agar can be poured into a plate without contamination. For plate 1, remove the cover to expose it to the lab atmosphere for allow for any airborne contamination. For plates 2, 3, and 4, choose a source of your choice. Rub the cotton swab on the selected place then use the swab to inoculate the agar plates. We selected a keyboard, a coin, and the lab door handle because we thought they would be good sources of bacteria.
Incubate these plates for one week. After incubation, identify and count the umber of mold and bacterial colonies that grew on each plate. The mold will appear fuzzy to the naked eye. We expect to find microorganisms on the plates labeled 1, 2, 3, and 4, but no microorganisms should appear on the sterile control plate. Results Our lab worked correctly and we found what we expected. We did not find any mold colonies, but we did find bacterial colonies. Figure 1 below is a representation of the bacterial colonies that was extracted from the air, a keyboard, a coin, and the door handle.
One week was given for the growth of the colonies. No bacterial growth was found on the sterile plate, but each of the other plates produced bacterial growth. The plate exposed to the air produced 1 bacterial colony, the keyboard had 20, the coin had 1, and the door handle had 5. Discussion We found bacterial colonies on each of the plates that were exposed to air and/or other possible sources of bacteria. Therefore, every source we used was contaminated with bacteria. We were able to find bacteria everywhere we looked.
These findings go along with what Tractor stated in his book that bacteria could be found practically everywhere (2010). We now know that extent to the number and locations of bacterial colonies, but how do they get on and in the human body? We acquire our initial microfilms at birth (Tractor, 2010). Some bacteria stay and make their home on the human body while others come and go. Bacteria can also get to the human body from objects we come in contact with, living or inanimate, from the air, or from vehicles such as food and water (Tractor, 2010). The large intestines house an extremely large number of bacteria.
This can be a source of potentially pathogenic bacteria that are in very close contact with areas of the body where hey could cause serious problems (Denatured, 1992). These potentially pathogenic bacteria, known as opportunistic bacteria, can be problematic when the bodes immune system is compromised or the bacteria get where they are not supposed to be. A common way the immune system’s microfilms can become compromised is by infection or with the use of antibiotics (Tractor, 2010). Antibiotics not only kill bad bacteria, but also good ones. When the delicate balance of bacteria is upset, opportunistic bacteria take advantage.
With compromised immune systems, the bacteria that make up the intestinal loran are responsible for many of the opportunistic infections that can transpire (Denatured, 1992). Escherichia coli is a good example of bacteria that is part of the normal microfilms in the large intestines, but if it is allowed access to other areas of the body like the urinary tract, it can be very problematic (Tractor, 2010). Since bacteria are found almost everywhere and the opportunities for them to become problematic are quite common, it is important for us to know how they can get on the human body, where they live, and ways to keep them in heck.
This experiment illustrated that bacteria can live and grow on many different surfaces. Bacteria can get on the human body in a number of ways and become problematic if they get in unwanted places or the immune system is compromised. This experiment worked well for bacteria, but not for other types of microorganisms like fungi, viruses, and protozoa. Also, one of our sources for bacteria should have been the human skin or in the mouth. This would have illustrated that we were able to isolate bacteria from our body in the lab setting.