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Procedure: I first labeled the sterile broth tubes and nutrient agar late with name, date, and organism I was inoculating with. Then I lit the Bunsen burner flame to sterilize the loop until the wire reached an orange hot point. I held the test tubes, agar plate and Escherichia coli tube at an angle to minimize airborne contamination, and swirled the sample of Escherichia coli. My partners’ samples were S. Erasures, B. Subsists, and M. Lutes. I transferred the microbe to the sterile tubes and flamed the tubes lip two or three times each.

Lastly I sterilized the loop and incubated the inoculated culture upside down at the signed temperature for the assigned time. Results: E. Coli had a great growth of yellow cloudiness with a few black specs in both tubes. The agar plate showed great growth as well with thick yellow lines of bacteria. My partner had S. Erasures and that showed a darker mustard color on the plate. Her tubes were cloudy with one of them developed brown spots at the bottom. The third bacterium was B. Subsists which had more growth than any other ones and this one had a grayish color to it.

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B. Subsists and M. Lutes had slight growth in both tubes; they had no big effect like the first two. Discussion and Conclusion: My results indicate that if transferring microbes without air Bourne contamination, the microorganisms will grow in the agar plate and tubes at the given temperature. A couple mistakes that my group had was not getting enough bacteria in the tubes. The two bacteria’s that showed little growth were B.

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