When Gram positive bacteria are treated with loosely, the loosely breaks down the pedagogical, allowing the cell to upturn if it is in a hypotonic solution or change shape without rupturing if it is in an isotonic solution. The ALPS of Gram negative bacteria protects the pedagogical from being affected by loosely. When Gram negative bacteria is treated with ethylene Dianne attractive acid (EDT), a collator, this destabilize the ALPS allowing it to become permeable.
The objective of this experiment was to demonstrate that loosely will cause bacteria cell walls to rupture, causing a decrease in population, depending on the similarity of its surroundings. Methods: In this experiment three groups of samples were made. The first and second group contained Gram positive bacteria. The first group contained a population of Microcosmic that had been grown slowly and was still growing. The second group contained a population of Microcosmic that had been grown rapidly and was in the stationary phase.
The third group contained a population of Gram negative bacteria. Each sample was placed in an HOPES buffer at a pH of 7. 2 and concentration of 1. 0 mm. This created a hypotonic solution. The first and second group contained three samples each. The first sample was a control and contained only the buffer and the Microcosmic. The second sample contained the buffer, the Microcosmic, and the loosely. The third sample contained the buffer, the Microcosmic, the loosely, and KICK used to create an isotonic solution. The third group contained five samples.
The first sample was a control and contained only the buffer and the Gram negative bacteria. The second sample contained the buffer, the Gram negative bacteria, and the loosely. The third sample contained the buffer, the Gram negative bacteria, the loosely, and EDT. The fourth sample contained the buffer, the Gram negative bacteria, and EDT. The fifth sample contained the buffer, the Gram negative bacteria, and a solution of KICK. Each sample was prepared with all the components except the loosely and an optical density reading was taken.
This was used to determine the size of the population at O time. After this reading was taken the loosely was added to the appropriate samples and timing began. Optical density readings were taken at 2 minutes, 5 minutes, 10 minutes, 15 minutes and 20 minutes. These optical onsite readings were used to track the effects of the loosely on the activity of the bacteria. Results: After all the optical density readings were recorded, each reading was divided by the initial reading at 0 time to get the fraction of the initial optical density reading.
This was used to show what percentage of the initial population remained at each recording. Gram positive bacteria Q represents the Gram positive bacteria sample in which only the loosely was added. R represents the Gram positive bacteria sample in which loosely was added in an isotonic solution. T represents the Gram positive bacteria sample in which only the loosely was added. U represents the Gram positive bacteria sample in which loosely was added in an isotonic solution.
Gram negative bacteria B represents the Gram negative bacteria sample in which only the loosely was added. C represents the Gram negative bacteria sample in which loosely and EDT was added. (Note the y-axis begins at . 089 to better illustrate the differences in sample populations. ) This chart illustrates the differences of how loosely affects Gram positive and Gram negative bacteria. Conclusions: This experiment demonstrated that when Gram positive bacteria was treated with loosely in a hypotonic solution the cells were lased and populations declined.
The loosely destroyed the pedagogical and allowed an inflow of fluid from the surroundings of the cells. When the Gram positive bacteria were in an isotonic solution, created by the addition of KICK, even though the loosely would have destroyed the pedagogical, there was no net inflow of fluid that would have caused the cells to else. The amount of decline in the Gram positive bacteria was dependent on whether the cells were growing or in a stationary phase.
The cells that were still growing showed less of a decline than the cells in the stationary phase. Since the growing cells are still dividing and increasing more rapidly in number than the stationary cells, which are in a phase where there is no net increase in cell numbers, the effects of cell death are seen less dramatically. With the Gram negative bacteria, the expected results were that the cells treated with EDT and loosely would have a greater decrease in population Han the sample that was only treated with loosely.
The DE TA destabilize the alphanumerically layer allowing the loosely to reach the pedagogical layer and destroy it. This allowed osmosis to occur in the hypotonic solution, lysine the cells. From the graph of the results this is seen to be the case. Gram negative bacteria have an extra layer of protection that protects it from its surroundings. This can be seen on the graph compared to the Gram positive bacteria. It can be seen that loosely has less of an effect on the Gram negative bacteria.