The purpose of this particular lab was to demonstrate the difference in separation of Benzene acid in water and Disintermediation. Also the difference in separation of Benzene acid in bicarbonate and Disintermediation. By calculating the partition coefficient for each of these separations, it became clear the differences in the separation of the aqueous and organic layers.
Observations & Results: In each of these separations, when the layer were mixed together and allowed to settle, two distinct layers were formed. The aqueous layer was on top and the organic layer was on the bottom. In both cases, gaseous pressure was built up in the separators funnel but in the bicarbonate separation, the gas build up was much greater due to the reaction giving off carbon dioxide. When each product was weighed, a white crystallized structure had formed in the Erlenmeyer flask.
Calculations: Partition Coefficient for Water K = . Egg organic layer / . 04 grams water layer 9. 0 Partition Coefficient for Bicarbonate K = . Egg organic layer / . Egg bicarbonate layer . 05 Conclusion: From this lab, we have learned that the partition coefficient for benzene acid can fifer greatly depending on what the aqueous layer in the separation is. For example, Benzene acid is soluble in disintermediation while only slightly soluble in water which leads to a large K value.
In the other experiment, the benzene acid reacts with the bicarbonate to form a salt and carbon dioxide. This reaction keeps the product mostly in the bicarbonate layer, therefore leading to a small K value.