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If true, write a “T” on the answer line. If false, write a word or phrase in the blank to make the statement true. 1) The microscope lens may be cleaned with any soft tissue. Gales. The microscope lenses should clean with a lens cleaner solution and a lens paper due to possible minerals or deposits on the any soft tissue. I | 2) The coarse adjustment knob maybe used in focusing with all objective lenses. False. The coarse adjustment knob should be used during scanning objective lens (lowest power). When it comes to higher objective lenses, the fine-focus knob should mostly be used and maybe a slight adjustment using the coarse adjustment knob.

I | 3) When beginning to focus, the lowest power lens should be used. T I | 4) Resolution decreases as the amount wavelength of light increases. T I | 5) When focusing always focus toward the specimen. T I B. Match the microscope structures in column B with the statements or phrases about them in column A. Column A I Column B I Answers I 1) Platform on which the slide rests for viewing I a. Incipience 5 2) Used for precise focusing after initial focusing b. Eyepiece 1 41 3) Used to increase the amount of light passing through the specimen I c. Stage | 1 | 4) Lens located on the superior end of the body tube I d. En focus knob 1 21 5) Carries the objective lenses and rotates to change magnification I e. Iris diaphragm 1 31 C. Explain the proper technique for transporting a microscope. You have to hold a microscope in upright position while one hand is on its arm and the other supports its base. When you set it down, try not to swing or jar it and put it down gently to prevent any damages to the microscope. D. Define the following terms 1. Field of view The area of the specimen being viewed through lenses 2. Resolution The ability of a microscope that differentiate two objects when you view them on a slide 3. Total magnification The magnifying power of the eyepiece multiply by the power of the objective lens. 4. Focal length The distance required for a lens to bring the light to a focus contrast 5. Contrast Contrast is defined as the difference in light intensity between the image and the adjacent background relative to the overall background intensity. The ability of a detail to stand out against the background or other adjacent details is a measure of specimen contrast. E. What happens to the size of the field of view as magnification increases? The size of the field of view decreases as magnification increases.

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When magnification increases, the field of view shrinks, which allows details in the magnified area to become better. F. What would be the total magnification of a specimen fifth eye piece is XX and the objective lens selected is XX? 15 X 4= power G. If observing a specimen on the low power objective, when switching to high power, the specimen is no longer visible. Why did this happen? There could be two possible reasons to why the view of the specimen disappears when you switch to higher objective lens. One is that the specimen could be slightly out of focus so you need to adjust the fine-focus knob.

Another reason is that the gyroscope cannot handle that much high-power from the objective lens the fine details cannot be processed at that level of power. Conclusions Why is proper microscope technique important for studying Anatomy and Physiology? Due to the limited ability of naked eye, a large part of anatomy and physiology is to study and look at the structures like cells and tissues. We need to know how to use and take care of a microscope not only for looking at specimens but also for safety reasons. Knowing a proper microscope technique will be a fundamental asset to study further about human bodies from the microscopic level.

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