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This is where the principle like dissolves like,” plays a factor in the separation of the dyes. Both molecules are relatively monopole but the size difference is what causes Red 40 to be a more polar molecule than Blue 1 because it is smaller. Introduction: In this lab, liquid chromatography is used to separate the dye colors of a Cool- Aid mixture. The mixture is injected into a chromatography column, which in this lab is a CO Seep-Pace. This is where the mixture lands on a substrate. The stationary phase may be polar or monopole. When a solvent is passed through the stationary phase, it will carry out the components of the same polarity.

This is where the principle ‘like dissolves like,” comes into play. This principle states that a polar substance will dissolve in a polar solution and vice versa, where a monopole substance will dissolve in a monopole solution. The speed that the substance comes out depends of the polarity of the substance and how well it dissolves in the solvent. Liquid chromatography is an essential tool when trying to determine what substances are used in materials we come in contact with every day. For example, such tool can be used to determine the substances in grape Cool-Aid.

Two known substances found in Cool-Aid is Red 40 dye and Blue 1 dye. These dyes have been linked to causing children to become hyperactive, and/or cause individuals to have an allergic reaction. This may cause someone to think twice before drinking grape Cool-Aid. Being able to separate these substances allows for individuals to determine if a material is safe for human consumption and handling. Materials: C 18 Seep-Pace Cartridge Two 10 ml Syringe with Male Lure Tip One 10 ml graduated cylinder Two 25 ml graduated cylinders 70% Sopranos (2-propane) Grape Cool-Aid Drink Mix (unsweetened) Distilled Water

Four 50 ml Beakers Three 100 ml Beakers Procedure: Step 1: Obtain goggles and prepared Cool-Aid mixture. Step 2: Prepare solvent and pre-treat C 18 Seep-Pace. Step 3: Inject Cool-Aid into Seep-Pace. Collect and discard effluent. Step 4: Elute the components of the grape Cool-Aid. Step 5: Record the volume of the ululate. Record the volume when the red dye first appears in the Seep-Pace and record again when the red dye is no longer present. Record the volume of the ululate when the blue dye is present and record again when the blue dye is no longer resent. Step 6: Repeat step 4-6 twice and record data.

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