Perform Benedicts test for reducing sugars. Introduction: Benedicts tests allows for the detection of the presence of reducing sugars. All inconsistencies are reducing sugars since all of them have active carbonyl group. Some disaccharide that are exposed to a carbonyl group are also reducing sugars but less reactive than inconsistencies. By mixing the sugar solution with Benedicts solution and heating them, a redo reaction will occur. The copper (II) sulfate present in Benedicts solution reacts with electrons from elderly or ketosis group of reducing sugars to form cuprous oxide, a red brown precipitate.
Materials: The materials used in order to detect starches was: test tubes, distilled water, Benedicts solution, starch were used. Negative Control: H2O Positive Control: Starch Obtain nine test tubes and number them 1-9 Add to each tube the materials to be tested. Add mi of Benedicts solution to each tube. Place all the tubes in boiling water- bath for 3 minutes and observe color changes during this time. After 3 minutes , remove the tubes from the water- bath and give the tubes ample time to cool to room temperature. Record the color of their contents.
Procedure 6. 2: Perform the iodine test for starch Tube Solution Benedicts Color Reaction Iodine Color Reaction 10 drops onion juice No change Blue->Black w/white 2 10 drops potato juice Precipitation Yellow-> blue 3 10 drops sucrose solution 5 10 drops distilled water 6 10 drops reducing sugar solution Blue 7 10 drops starch solution Yellow-> Blue 8 Unknown Blue-> Red Brown 9 Unknown 2 4 10 drops glucose solution.