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Cellular respiration lab Procedure Materials: 2 water baths 2 rulers 2 thermometers h ml of germinated seeds (in each syringe) 2 syringes h ml non-absorbent cotton (in each syringe) h ml absorbent cotton (in each syringe) Kayo (3 drops in each) 2 capillaries tubes 4 washers (2 in each syringe) Tape Soap-nanometer fluid (one drop in each syringe) Steps: For each respirator 1 . Glue the capillary to the syringe making sure there are no leaks and that it is not clot 2.

Put 1/ml of absorbent cotton inside the syringe .Put about 3 drops of Kayo in the cotton using another syringe without touching the walls of the respirator 4. Put h ml of non-absorbent cotton 5. Put h ml of germinated seeds. Push the plunger into ml For water bath 1. Attach a ruler to the bottom of the water bath using tape 2. Fill with water 3.

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Put ice on one of the water baths until it reaches 10 Co. Measure the temperature with the thermometer 4. Place a piece of tape horizontally in each water tube 5. Put 2 washers on each respirator.Submerge the respirator making sure the body is completely covered with water while the tip is resting on the piece of tape. Wait for 5 minutes 6. Put a drop of nanometer fluid (soap) on the capillary tube using a syringe with a needle 7.

Submerge the whole microdensitometer in each water bath 8. Every 5 minutes, for a total of 25 minutes, record how much the nanometer fluid has moved by using a magnifying glass, in reference to the ruler attached at the bottom of the water bath. 9.Make sure the temperature is kept the same Variables Manipulated variable I Responding variable I Control I Constant I Temperature (10 Co)l Rate of respiration (measured by the difference between initial value and final value in the ruler) | 19 Co water I * Germinating seed (0.

Ml) * 0. Ml absorbent cotton * 0. Ml non-absorbent cotton *3 drops Kayo (in each syringe) * Kayo concentration (15%) *Time (5 minutes) * Total time (25 minutes) * 5 minutes of equilibration * amount of nanometer fluid (2 drops) I

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