Ethanol from Sucrose * Introduction Ethanol is one of the oldest alcohols and also the least toxic one. Industrially, ethanol is made most economically by hydration of ethylene. However, ethanol that is intended for human consumption must, by law, be prepared by fermentation. By either method, ethanol, of course, has the same formula, structure, and properties. The fermentation takes place with the assistance of enzymes from yeast in 2 steps: first sucrose (a disaccharide whose structure is n your textbook) is broken down to two incarcerations, glucose and fructose.
These incarcerations are then converted into ethanol and carbon dioxide. Materials 250-ml Erlenmeyer flask 250-ml Beaker Brunched funnel Fermentation apparatus Distillation apparatus Vacuum filtration assembly Water Sucrose Potassium phosphate Celtic Disposed hydrogen phosphate Dried baker’s yeast * Procedure Part I Place 20. 0 g of sucrose into a 250-ml Erlenmeyer flask, add 100 ml of water, and shake until sucrose is completely dissolved. To the solution add 0. 50 g of sodium hydrogen phosphate, 1 g of potassium phosphate, and 2 g of dried baker’s yeast.
Mix the contents thoroughly by shaking the mixture. Set up the apparatus for the fermentation, label it, and leave it under 30-35 degrees Celsius for 5-7 days. Part II Remove the rubber stopper from the flask without disturbing the solution. Prepare a vacuum filtration assembly. Into a 250-ml beaker, place 100 ml of water and one table spoon of Celtic. Stir and pour mixture into the Brunched funnel while the water is running and a vacuum is applied. Discard the water collected.
Carefully decant the liquid in the fermentation flask above the sediment through the Celtic filter aid. Obtain a distillation setup, assemble it, and place the solution. Heat up the liquid gradually until it starts to boil. When the temperature reaches 78 degrees Celsius, begin to collect the solution. Stop once the temperature has reached 90 degrees. * Conclusion The experiment was partially successful because we obtained the ethanol. However, the ethanol obtained was not completely pure. I think that was due to some error in the assembly of the apparatus.