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Two additional samples were made; one was blank and the other was for the specific homogenate sample. Then 3 micrometers of Bradford assay reagent, which indicates the amount of protein present by color, was added to all samples. The spectrophotometer was zeroed at 595 NM. A standard curve was made with the different absorbency and concentrations. After the linear equation was formed, the unknown sample concentration was determined using the standard curve equation. A Gel Electrophoresis was used to perform a qualitative analysis.

The use of 5 micrometers of the homogenate was heated to 80 degrees Celsius. Then the homogenate was transferred to a 2-micrometer-protein gel sample buffer. Samples loaded on to the gel was run at 100 v and stained with comatose blue; observations were made next lab. (Gladdening 2014) The amount of glucose and glycerol were determined from the homogenate sample as well. Samples A(+) and B(-) were used to determine glucose. Sample A had OMG/ml maliciousness’s in . MM citrate buffer, pH 5. 0 and was incubated at 370 Celsius for two hours to allow for the enzyme to digest the glycogen.

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This concentration was multiplied by two to account for the dilution. The liver had the most glycogen concentration (2. 499 MGM/ml) compared to the heart(0. 684 MGM/ml) and kidney(0. 904 MGM/ml)(Figure 4) 011176000 Figure 4. Concentration of glycogen is shown in the heart, kidney, and liver homogenates of boss Taurus. Errors bars were created from 5 readings for heart, 6 readings for liver, and 7 readings for kidney. Free glucose were more abundant in the liver with a concentration of 1. 451 MGM/ml compared to the heart (. 3129 g/ml) and kidney (. Magma/ml).

Free glucose concentrations were determined by using the non-enzyme treated sample. Using the standard curve equation, concentration was found and multiplied by 2 to account for dilution. Figure 5 Free glucose concentrations of heart, kidney, and liver homogenates of boss Taurus. Errors bars were created from 5 readings for heart, 6 readings for liver, and 7 readings for kidney. Figure 4 and 5 both indicated a similar pattern. The liver for glycogen had a concentration of 2. 499 MGM/ml and a free glucose concentration of 1. 451 MGM/ml. When compared to the heart and liver, the heart

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