Another loop-full tot bacteria was added to the test tube intonating S ml of sterile saline, recapped and shaken by flicking, S ml of water was added to the bottom of the incubation tray to provide a humid atmosphere. A Pasteur pipette was then filled with a bacterial suspension, The incubation tray was tilted and tubes GNP, TAD, MIND, GLIB, MAN, ION, SORE, RAH SAC, MEL, AMY and AR were filled with the bacterial suspension. The microbes DAD, OLD, DOC, HAS and LIRE were slightly undefiled. 10th the tube and cupules sections of CIT, UP and GEL tubes were filled. After inoculation, the cupules sections of DAD, OLD, DOC, HAS and ERE were filled vita mineral oil to create an anaerobic condition.
The lid was then placed on the incubation tray and incubated at 37 degrees Celsius for 18-24 hours. An isolation streak was made on the TTS plate With a portion Of the bacterial suspension to ascertain the purity Of the suspension.After 18-24 hours of incubation, all reactions not requiring addition of reagents were observed and recorded. The microbes that required additional reagents had them added and time allotments were fulfilled to get results of remaining tests. The seven-digit profile number was then determined after all reactions were recorded on the report sheet and the profile number of the enteric unknown was calculated.The identity of the enteric unknown was then looked up by using the API EYE software on the computer. Results: [pick] [pit] [pick Epic] +1 Discussion: In reading the API 20 E there are many different reactions tested such as a beta-glaciological, argentine dehydrators, lysine destroyable, citrate utilization, Ernestine destroyable, citrate utilization, HAS production, urea hydrolysis, demeans, indolence production, action production, gelatins, fermentation/oxidation and oxides.
The strip is read by noting color changes after the various indicator systems have been affected by the metabolites or added reagents. Conclusion: In conclusion, the unknown enteric bacterium was identified as Seriate mercenaries with a seven-digit profile number of 7307741. The Nitrate reduction test was negative for gas therefore positive for Nitrate reduction. The Catalane test was also positive breaking down the H2O into 02.