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Like all catalysts, enzymes work by lowering the activation energy for a reaction, thus aromatically increasing the rate of the reaction, As a result, products are formed taster and reactions reach their equilibrium state more rapidly. Most enzyme reaction rates are millions of times faster than those of comparable urn-catcalled reactions. As with all catalysts, enzymes are not consumed by the reactions they catalyst, nor do they alter the equilibrium of these reactions. However, enzymes do differ from most other catalysts in that they are highly specific for their substrates.

Enzymes are known to catalyst about 4,000 biochemical reactions. This activity/lab displays how enzymes work, and shows the effects of different substrate concentrations on the rate of enzyme activity The toothpicks will break toothpicks as instructed, while the time keeper watches the clock. This breaking of toothpicks in a set amount of time represents how enzymes speed up reactions. We hypothesize that the toothpick’s rate of activity will be higher when the toothpicks are more concentrated, as opposed to when they are less concentrated.

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If the toothpicks rate of activity is higher when the toothpicks are more concentrated, then enzymes work faster when substrates re more concentrated. Materials 1. Two groups of 50 toothpicks, and one group of 35 toothpicks per person 3. A watch or clock with easy access 4. One plastic bag 5. One lab packet per person 6. One calculator (optional) procedure 2. One pencil First, you must gather all the materials needed to successfully perform the lab. Next, clear a clean, medium-sized work space and remove all your toothpicks from your plastic bag.

Then, divide your toothpicks into two groups of 50 and one group of 35 (make sure they are separated, so the toothpicks don’t accidental ix). After that step is completed, take one of your groups of 50 toothpicks, and put it in front Of the toothpicks_ Now, have the toothpicks break toothpicks with one hand, while the time keeper puts 10 seconds on the clock. When the ICC seconds is completed, the time keeper must tell the toothpicks to Stop breaking toothpicks. *Note- the broken toothpicks MUST NOT BE REMOVED from the toothpick pile* Record how many toothpicks were broken, and insert the data into the correct table.

Repeat the previous steps concerning the breaking of he toothpicks, but instead have the time keeper put 20, 30, and 60 seconds on the clock, consecutively. After this is completed, also record this data, and insert into the correct table. *Note. If you run out of toothpicks before the time is up, record the amount of time it took to break the remaining toothpicks, and insert it into the correct table* Now that Part A is completed, move on to Part a. Repeat everything in part A but now spread out the toothpicks so that the toothpicks has to reach tort them (toothpick concentration decreases), and use the group of 35 toothpicks.

AS before, record all data and insert it into the correct table, After completing Part a, move onto Part C. Repeat everything in Part A but now have both partners break the toothpick (double enzyme concentration), Again, record all data and insert it into the correct table. Now that the lab activity is over, perform the calculations given at the bottom of page 2, and insert that data into the correct tables. Finally, clean up, write a conclusion, and answer the post-lab questions. Data Table 1. Toothpicks Activity Table 2. Rate of Toothpicks Activity Table 3. Toothpicks Activity (Decreased Toothpick Concentration) Table 4.

Rate of Toothpicks Activity (Decreased Toothpick Concentration) Table 5. Toothpicks Activity (Double Enzyme Concentration) Table 6. Rate of Toothpicks Activity (Double Enzyme Concentration) Analysis Total Toothpicks Broken vs.. Cumulative Time Seconds/Tips Broken Seconds Broken Calculations: The graph above the calculations table shows both the cumulative seconds passed, and the total number of toothpicks broken within those seconds. This also shows that the number Of toothpicks broken rose dramatically within the iris 30 seconds or so, and then the breaking rate slowed down.

This means that either the toothpicks ran out Of toothpicks before time was called, or he/she got tired, which slowed their breaking speed. Questions: 1. The reaction rate slowed down as the supply of unbroken toothpicks diminished because there was too much time allotted to break the small amount of unbroken toothpicks that remained. 2. The reaction rate would steadily increase, then peak and remain steady because the toothpicks has a limited rate for breaking toothpicks, and cannot physically break faster past a certain mint. 3.

The reaction rate skyrocketed when two toothpicks were working because two “enzymes” working together to break down substances work faster than one working on its own. 4 The reaction rate of the spread out toothpicks slightly rose because the toothpicks could grab individual toothpicks easier then when they were all in one giant clump. 5. I believe that the reaction rate of the separated products and reactants would rise because then the enzyme wouldn’t have to deal with all the products getting in the way while it was trying o break down a reactant, 6.

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