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All of the plates showed thick white colonies for the SCM-Trap plates.

They should have been red because the Dade colony streaked on the plate was red. They were still white because the adenine from the plate had not been exhausted. However, the cells are Dade- because they are red on all the other replica plates. The Scheme plates are also a control. In order to ensure that the cells are Dade-, they would not be able to grown on this plate or have sparse red colonies.

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Because the cells have the dysfunctional tort of the Dee gene, they would not be able to survive with adenine present in the plate, Since YAP plate was the last replica plate, it was used as a control to make sure that the transfer was effective for all the other plates. Since red colonies appear red on all the other media plates, it is concluded that the transfer was effective. Results: Growth on the replica plating Plate 1121 3141 SCM-Rag Red colonies I Red I Red Red I SCM-Dad I Sparse Red I NO growth Sparse Red Sparse Red SCM-His Sparse Red I Red Red I Red SCM-Trap White White I White White IYIPPY Red I Red I Red Red I Conclusion: We plated the red colonies or sectors on Cascara, SCM-His, and Swiss to discern the genotype of each colony or sector.

Colony 1 is Adele hiss because of the sparse red growth seen on the SCM-Dad and Swiss plate. Colony 2, 3 and 4 are Dade. The colonies on the SCM-Trap plates were white but should have been red. They were white because they haven’t exhausted the adenine present in the plate. Many of the Dade cells were also hi because the recombination occurred between the Dade gene and controvert.Since mitotic recombination is rare, the chance of double recombination is improbable so the cells would RAGA. Further Questions: Yeast can also exist in haploid form.

So the Dade hiss raga genotype is possible if there is only one copy of the chromosome and there is loss of the chromosome that was build type for all the genes. Ii. Red sectors in white colonies arise mitotic recombination, by loss of the entire chromosome containing the Dade+ allele, by a deletion Of the portion Of the chromosome containing the Dade+ allele, or by spontaneous mutation of the Dade+ allele to an Dade- allele. Ii.

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