There are many factors that will affect the reaction rate of the enzymes, that produce hydrogen reside into oxygen and in the pig liver: pH, temperature, pressure, and surface area of the pig liver. How does the temperature affect the reaction rate of enzymes in the pig liver, which produce hydrogen peroxide into hydrogen and oxygen?
Aim: To find out how does the temperature affect the reaction rate of enzymes in the pig liver, which produce hydrogen peroxide into water and oxygen, by adding hydrogen peroxide of different temperature into minced pig liver, and timing the time that it takes for the enzymes in the pig liver to create ml of oxygen gas. Hypothesis: hind that the enzyme’s reaction rate will be the highest at around ICC, animal enzymes will start to denature above temperatures of ICC, the normal body temperature of pigs, and work quickest (Worthington, n. . )The body temperature is regulated at such a temperature to allow metabolism to happen at a highest rate. If the temperature is higher than ICC, then the enzymes will denature and will no longer work; if the temperature is lower than 38. ICC then the reactions will not have enough energy to take place, and you will have hypothermia. Hence, think that the results would from a mountain like curve, as the reaction rate old decline as the temperature increased or decreased father from the pig’s normal body temperature range.
Apparatus: Thermometer Measuring Cylinder Rubber tubing Water bath Test tube Measuring Scale Test tube Stand Gas Collector Water Tub Minced Liver Rubber Plug Hydrogen Peroxide – Thermometer – Gas Collection Kit – Test tube with hole – Rubber Tubing – Gas collector – Water Tub – Test tube stand – Liver – Spatula – Electronic Balance – Stopwatch – Measuring Cylinder – Beaker – Hydrogen Peroxide (1% concentration) – Water bath (20-ICC) Method: Step 1: Use a spatula and take minced pig liver and put it onto the scale, over a issue, which mass is rested to zero before hand, then measure 0. Gram, and take that one gram of minced pig liver and place it into the test tube. Step 2: Connect the rubber tubing to the test tube and to the gas collector, which is on the box of the gas kit, as shown in the apparatus Step 3: Clam the test tube into the the test tube stand. Step 4: Fill the water tub with container with water, until it is well above the gas collector (1 CM about).
Step 5: Fill another measuring cylinder (measures at least to ml) with water in the gas kit box, while emptying the oxygen inside, then while keeping the top f the measuring cylinder underwater, flip the measuring cylinder upside down onto the hole of the gas collector. Step 6: Measuring with a measuring cylinder, pour mm’ of 1% concentrated hydrogen peroxide into a beaker.
Step 7: Place the beaker in a water bath, which is set to heat things up to ICC (the temperature is presented on the water bath switch), then a thermometer into the beaker, and heat the beaker for a while, then record the temperature on the thermometer, for the water bath temperatures are inaccurate. Step 8: Pour the heated hydrogen peroxide into the test tube, while quickly tarring the timer, once the acid touches the minced liver, start the stop watch and quickly putting a stopper onto the test tube. Then stop the stop watch once the measuring cylinder, which is on top of the gas collector, has collected ml of gas.
Step 9: Then record the the time that it takes for the enzyme to produce ml of oxygen gas with a particular temperature of hydrogen peroxide. Step 10: Redo step one to eight, but instead heat/cool the temperature of hydrogen peroxide to different 6 temperatures. Cool the hydrogen peroxide with a bath, which has ice in it. Fair Testing: Control How Control Why Control The PH surrounding the enzyme. Consistently use a single type of acid of the same concentration. The pH will affect the structure of the enzyme, as a pH level too far from the enzyme’s optimum pH will cause it denture.
To control that we must constantly use a single type of acid, means that the same rate of H+ ions, which makes acid acidic, are released; and a same concentration of that acid means that the concentration of H+ ions the same. The pressure of environment during the reaction. Make sure that the acid height on top of the minced liver is the same. Higher the pressure then the particles are bouncing off each other with more force; hence, reactions will happen quicker and more frequently. Keeping sure that the height of the acid on top of the minced liver is the same, makes sure that the force acting on the minced liver is the same.
However at the same time you also have to make sure that the surface area of the pig liver is the same (Although this will not affect the trails greatly) Surface area of pig liver. Used minced pig liver, and make sure all of them are flat on the bottom of the test TU be. Although the surface area of the pig liver will not affect the enzyme’s individual action rate; however, it is impossible for us to measure enzymes’ reaction rate individually. The enzymes on the surface of the pig liver will get to react with the hydrogen peroxide first, so the larger the surface area, then the faster the enzymes will react.