Amylase causes a chemical reaction in the polysaccharide starch that breaks down the glucose molecules into maltose. When diluted with a solution of KAKI, starch will change to a dark purple while doing the same to maltose, there would be no color change. The question posed, does more or less concentration speed up the reaction rate? Group 11 predicts that the more concentrated solution of amylase (1>5), the reaction rate will be faster; therefore test tube 1 will have the quickest reaction rate.
The rate of disappearance of starch in amylase can be hon.. By diluting amylase in a 1:1 ratio but using Tube 1 to create Tube 2, Tube 2 (a mix of 1 and 2) to create Tube 3 and so on for 5 Tubes. Materials test tube rack 10 standard test tubes wax pencil test plate flask of distilled water beaker of distilled water ml graduated cylinder 1 calibrated ml pipette 2 calibrated ml pipette disposable Pasteur pipettes pipette bulb buffer solution kill solution 1% starch solution 1% amylase solution Methods The lab group prepared amylase dilution in the first set of test tubes (1-5). Ml amylase was added to a test tube already containing ml distilled water creating the first tube to have . 5% amylase. Next ml of amylase from test tube 1 was added to the ml of distilled water in test tube 2, giving test tube 2 a dilution of . 25%. This was continued for test tubes 3, 4, and 5 giving them dilutions of . 125%, . 063%, and . 031% amylase respectively. The experimental test tubes (la-AAA) were then created by transferring ml of dilution from test tube 5 to test tube AAA, and so on for (la-AAA).
Test tube 1 and 1 a were no longer used after the experimental et was prepared. 40 drops of pH 6. 8 buffer solution is added to each tube in the experimental set and mixed. Next, 1 or 2 drops of kill were added to each compartment of the four rows on a test plate. A separate row was used for each concentration of amylase. Finally, it was ready to be tested. 1 Ml of 1% starch solution was added to tube AAA and mixed, time was recorded immediately at this point. Next 1 drop of the mixture from tube AAA was quickly added to the drop of kill in in the first compartment of the test plate.
The reaction mixture was hen sampled at 10-second intervals using a new compartment each time. When the blue color was no longer produced, the kill remained yellow-amber which showed that all of the starch went through digestion. Time was recorded on a table and the steps were repeated for the other concentrations. Results % Amylase I Time of Starch Disappearance (in minutes) “0. 25” 19 1 “0. 125” 112 1 “0. 063” 20 | I none “0. 031” Discussion I “so’ 14 The group hypothesized the the more concentrated tube of amylase, the faster the rate of reaction would be.