These crosses were then incubated until week two. In week two, the parents were removed and disposed of. The eggs and larvae were left to continue the cross. The crosses were left to continue to incubate until the following week. In week three, the flies emerging from each cross of the Pl generation were counted, separated into male and female, and then further separated into phenotype. This marked the completion of the Pl generation. The Fl crosses were then begun. They were set up in there different ways.
In Cross A, Fl females were paired with Fl males. In cross B, Fl virgin females were paired with dumpy sepia males. In the last cross, Cross C, dumpy sepia virgin females were crossed with Fl males. In crosses B and C, only virgin females were used. The virgin females used were obtained from the Pl crosses. Dumpy sepia virgins were acquired from a source outside the experiment. The crosses were left to incubate until week four. Week four proceeded much like week two, with the arenas being removed and discarded.
The eggs and larvae were left undisturbed to continue the cross. They were allowed to incubate until the final week, week five. In the final week, week five, the flies emerging from the IF crosses and the backdrops were counted, separated into male and female, and then further separated into phenotype just as week three.