Background:Garlic(Allium sativum) is used as a common household medicine for the treatment of abdominal discomfort, diarrhea, otitis media and respiratory tract infections.
The garlic oil is known to posses’ antimicrobial and antioxidant potentialObjective:To study the effect of garlic oil on the nickel chloride induced somatic and germinal cell nuclear damages in miceMethods:Commercially available garlic oil was used to study the effect on nickel chloride (10 mg/kg, i.p) induced nuclear damages. Three doses of garlic oil viz.
, 50, 100 and 200 mg/kg, i.p was tested. Peripheral blood micronuclei and sperm shape abnormalities were analyzed to determine the somatic and germinal cell damages, respectively. The incidences of micronuclei were evaluated in two types of erythrocytes such as polychromatic erythrocytes (PCEs) and normochromatic erythrocytes (NCEs). The data from the studies were compared between groups by using the student t test and Anova.Results: The data obtained from the study indicated that nickel chloride treatment significantly (p<0.01) reduced the population of PCEs and NCEs, besides increasing the frequency of micronuclei in these cells and enhanced the sperm abnormalities compared to the control group.
Administration of garlic oil at higher dose (200 mg/kg) produced significant enhancement in the number of NCEs and reduced the number of micronuclei in these cells. The inhibitory effect was also observed in the sperm shape abnormalities. These effects were found to be feeble when garlic oil was tested at 50 mg/kg. However, the group that received 100 mg/kg produced total mortality in the animals.?Conclusion: The observation from the present study indicated that garlic oil prevented the incidences of nuclear damages in both somatic and germinal cells induced by nickel chloride, however, more research is needed to support precise inference.Key word: Garlic oil, Nickel chloride, nuclear damage, micronucleus, sperm abnormality.